Coumarin-SAHA (CAS 1260635-77-5)

Coumarin-SAHA is a SAHA derivative where the anilino “cap” group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC. The fluorescence excitation and emission maxima of free c-SAHA is 325 and 400 nm, respectively and is quenched by 50% when bound to HDAC8. This probe can be used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes and is well-suited for high-throughput screening.